Journal: Oncology Reports

Article Title: Forkhead box F1 functions as a novel prognostic biomarker and induces caspase‑dependent apoptosis in bladder cancer

doi: 10.3892/or.2023.8610

Figure Lengend Snippet: FOXF1 expression is downregulated in tumor tissues and is associated with a poor prognosis of patients with BC. (A) Expression of FOXF1 in pan-cancer and (B) BC tissues in the Ruijin Cohort. The association of the FOXF1 expression levels with (C) tumor type, (D) pathological grade, (E) muscle invasion, (F) T stage and (G) American Joint Committee on Cancer stage in the Ruijin Cohort. (H) The H-score curve and (I) survival status of FOXF1-low group and FOXF1-high group in the Ruijin Cohort; patients were listed in an order of increased FOXF1 expression level; the dotted line represents the cut-off value between the two groups. (J) Kaplan-Meier analysis of overall survival in the Ruijin Cohort and (K) in the GSE48075 dataset. FOXF1, forkhead box F1; BC, bladder cancer; ACC, adrenocortical carcinoma; BLCA, bladder urothelial carcinoma; BRCA, breast invasive carcinoma; CESC, cervical squamous cell carcinoma and endocervical adenocarcinoma; CHOL, cholangiocarcinoma; COAD, colon adenocarcinoma; DLBC, lymphoid neoplasm diffuse large B-cell lymphoma; ESCA, esophageal carcinoma; GBM, glioblastoma multiforme; HNSC, head and neck squamous cell carcinoma; KICH, kidney chromophobe; KIRC, kidney renal clear cell carcinoma; KIRP, kidney renal papillary cell carcinoma; LAML, acute myeloid leukemia; LGG, brain lower grade glioma; LIHC, liver hepatocellular carcinoma; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; OV, ovarian serous cystadenocarcinoma; PAAD, pancreatic adenocarcinoma; PCPG, pheochromocytoma and paraganglioma; PRAD, prostate adenocarcinoma; READ, rectum adenocarcinoma; SARC, sarcoma; SKCM, skin cutaneous melanoma; STAD, stomach adenocarcinoma; TGCT, testicular germ cell tumors; THCA, thyroid carcinoma; THYM, thymoma; UCEC, uterine corpus endometrial carcinoma; UCS, uterine carcinosarcoma.

Article Snippet: Subsequently, the microarray was incubated with primary antibody against human FOXF1 (1:100 dilution; cat. no. PAB30083, Abnova) overnight at 4°C.

Techniques: Expressing

Journal: Oncology Reports

Article Title: Forkhead box F1 functions as a novel prognostic biomarker and induces caspase‑dependent apoptosis in bladder cancer

doi: 10.3892/or.2023.8610

Figure Lengend Snippet: Association between FOXF1 expression and the clinicopathological characteristics of patients with BC.

Article Snippet: Subsequently, the microarray was incubated with primary antibody against human FOXF1 (1:100 dilution; cat. no. PAB30083, Abnova) overnight at 4°C.

Techniques: Expressing

Journal: Oncology Reports

Article Title: Forkhead box F1 functions as a novel prognostic biomarker and induces caspase‑dependent apoptosis in bladder cancer

doi: 10.3892/or.2023.8610

Figure Lengend Snippet: dsFOXF1-367 induces FOXF1 expression in human BC cells. The mRNA expression level of FOXF1 was downregulated in (A) bladder tumor tissues and (B) BC cells lines. (C) The protein expression levels of FOXF1 in T24 and EJ cells were assessed using western blot analysis; GAPDH served as the loading control; (D) the relative FOXF1 protein expression levels were quantified by determining the gray value. (E) Subcellular expression of FOXF1 protein (red) in T24 and (F) EJ cells was detected using immunofluorescence (magnification, ×400); nuclei were stained with DAPI (blue). *P<0.05, **P<0.01 and ***P<0.001, vs. respective control. FOXF1, forkhead box F1; BC, bladder cancer.

Article Snippet: Subsequently, the microarray was incubated with primary antibody against human FOXF1 (1:100 dilution; cat. no. PAB30083, Abnova) overnight at 4°C.

Techniques: Expressing, Western Blot, Control, Immunofluorescence, Staining

Journal: Oncology Reports

Article Title: Forkhead box F1 functions as a novel prognostic biomarker and induces caspase‑dependent apoptosis in bladder cancer

doi: 10.3892/or.2023.8610

Figure Lengend Snippet: dsFOXF1-367 suppresses cell proliferation, migration, invasion and induces apoptosis in BC. CCK-8 assays were used to examine the viability of (A) T24 and (B) EJ cells. EdU assays revealed that dsFOXF1-367 inhibited (C) T24 and (D) EJ cell proliferation. The replicating DNA were marked with Apollo567 (orange), while nuclei were stained with DAPI (blue; magnification, ×200). (E) Proportion of replication DNA in T24 and EJ cells. (F) dsFOXF1-367 impaired the clonogenic capacity of T24 and EJ cells; (G) the colony formation rate in each group was compared in the histogram. (H and I) Representative images (magnification, ×200) of Transwell assays (H) with or (I) without Matrigel in T24 and EJ cells; the numbers of (J) migrated and (K) invaded cells were compared. Flow cytometry of (L and M) T24 cells following transfection with (L) dsControl or (M) dsFOXF1-367, and (N and O) EJ cells following transfection with (N) dsControl or (O) dsFOXF1-367. Percentages of (P) T24 and (Q) EJ cells in early and late apoptosis. (R) The heatmap revealed 1,178 protein coding genes among 34,715 genes which were possibly regulated by FOXF1 activation. (S) Protein-protein interaction network of potential hub genes and (T) the interaction degree of top 10 genes. (U) The protein expression levels of apoptosis-related genes. *P<0.05, **P<0.01 and ***P<0.001, vs. dsControl. FOXF1, forkhead box F1; BC, bladder cancer.

Article Snippet: Subsequently, the microarray was incubated with primary antibody against human FOXF1 (1:100 dilution; cat. no. PAB30083, Abnova) overnight at 4°C.

Techniques: Migration, CCK-8 Assay, Staining, Flow Cytometry, Transfection, Activation Assay, Expressing

Journal: Oncology Reports

Article Title: Forkhead box F1 functions as a novel prognostic biomarker and induces caspase‑dependent apoptosis in bladder cancer

doi: 10.3892/or.2023.8610

Figure Lengend Snippet: Promoting effect of FOXF1 on the apoptosis of T24 and EJ cells is reversed by caspase-3 inhibitor. Apoptosis assays of T24 cells treated with (A) dsControl, (B) dsControl plus caspase inhibitor, (C) dsFOXF1-367 and (D) dsFOXF1-367 plus caspase inhibitor, respectively. (E) Percentage of T24 cells undergoing apoptosis and (F) cell viability in each group. The apoptosis assays of EJ cells treated with (G) dsControl, (H) dsControl plus caspase inhibitor, (I) dsFOXF1-367 and (J) dsFOXF1-367 plus caspase inhibitor, respectively. (K) Percentage of EJ cells undergoing apoptosis and (L) cell viability in each group. **P<0.01 and ***P<0.001, vs. dsControl or as indicated. The cell apoptotic rates in the dsFOXF1-367(+) Ac-DEVD-CHO(−) groups were compared with those in the dsControl(+) Ac-DEVD-CHO(−) groups, and the cell apoptotic rates in the dsFOXF1-367(+) Ac-DEVD-CHO(+) groups were compared with those in the dsFOXF1-367(+) Ac-DEVD-CHO(−) groups, respectively. FOXF1, forkhead box F1.

Article Snippet: Subsequently, the microarray was incubated with primary antibody against human FOXF1 (1:100 dilution; cat. no. PAB30083, Abnova) overnight at 4°C.

Techniques:

Journal: Oncology Reports

Article Title: Forkhead box F1 functions as a novel prognostic biomarker and induces caspase‑dependent apoptosis in bladder cancer

doi: 10.3892/or.2023.8610

Figure Lengend Snippet: Activation of FOXF1 suppresses the tumorigenesis of EJ cells in vivo . (A) The procedure of injection, weighting and measuring of xenograft tumor models. (B) EJ Lenti-dsControl and EJ Lenti-dsFOXF1-367 cells (6×10 6 , 200 µl) were injected into the right flanks of the mice; the mice were examined for 30 days. (C) Subcutaneous tumors in each group were dissected and photographed. (D and E) Body weights and tumor volumes in each group. *P<0.05 and **P<0.01. The image in (A) was created using BioRender.com. FOXF1, forkhead box F1.

Article Snippet: Subsequently, the microarray was incubated with primary antibody against human FOXF1 (1:100 dilution; cat. no. PAB30083, Abnova) overnight at 4°C.

Techniques: Activation Assay, In Vivo, Injection

Journal: Oncology Reports

Article Title: Forkhead box F1 functions as a novel prognostic biomarker and induces caspase‑dependent apoptosis in bladder cancer

doi: 10.3892/or.2023.8610

Figure Lengend Snippet: The mechanisms of the antitumor signaling of dsFOXF1-367 in bladder tumors. The figure was created using BioRender.com. FOXF1, forkhead box F1; Ago 2, Argonaute 2.

Article Snippet: Subsequently, the microarray was incubated with primary antibody against human FOXF1 (1:100 dilution; cat. no. PAB30083, Abnova) overnight at 4°C.

Techniques:

Journal: Oncotarget

Article Title: A novel microfluidic model can mimic organ-specific metastasis of circulating tumor cells

doi: 10.18632/oncotarget.9382

Figure Lengend Snippet: ( A – C ) Typical metastatic colonies of MCF-7, MDA-MB-231, and ACC-M cells after HE staining and anti-human Pan CK immunostaining in the lung of nude mice. The metastatic colonies are circumscribed by black dotted lines and indicated by asterisk. Scale bar = 50 μm. ( D ) The metastasis rates and the colony numbers of MCF7, MDA-MB-231, and ACC-M, respectively. ( E ) The metastasic colony areas of MCF-7, MDA-MB-231, and ACC-M cells. The colony areas of MDA-MB-231 cells were significantly higher than those of MCF-7 cells (** p < 0.01, n = 5 per group).

Article Snippet: Immunohistochamical staining with anti-humna Pan CK (Merckmillipore, USA) was performed to detect the metastatic colonies.

Techniques: Staining, Immunostaining

Journal: Biomolecules

Article Title: Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters

doi: 10.3390/biom12121769

Figure Lengend Snippet: Immunohistochemical expression of galanin receptors (GALR1, GALR2, and GALR3) in myenteric plexuses (MP) distantly located from the colorectal cancer (CRC) tissue compared with their expression in the myenteric plexuses in the vicinity of cancer invasion of the same, representative CRC patients (n = 31). Neurons expressing respective GALRs are marked by arrows. Total magnification: 400× g .

Article Snippet: The sections were incubated overnight at 4 °C with rabbit polyclonal anti-human antibodies against GALR1 (Cat. No. GTX108207, GeneTex, Irvine, CA, USA), GALR2 (Cat. No. GTX100382, GeneTex, Irvine, CA, USA) and GALR3 (Cat. No. GTX10816, GeneTex, Irvine, CA, USA), all diluted 1:400 in PBS.

Techniques: Immunohistochemical staining, Expressing

Journal: Biomolecules

Article Title: Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters

doi: 10.3390/biom12121769

Figure Lengend Snippet: Immunoreactivity (IR) of galanin receptors (GALR1—( A ), GALR2—( C ), and GALR3—( E )) in myenteric plexuses (MP) distantly and proximity located to cancer invasion in individual colorectal (CRC) patients. The average immunoreactivities of GALRs (GALR1—( B ), GALR2—( D ), and GALR3—( F )) in myenteric plexuses (MP) located distantly from CRCcells were compared with those in plexuses in the vicinity of cancer cells of colorectal (CRC) patients (n = 31).

Article Snippet: The sections were incubated overnight at 4 °C with rabbit polyclonal anti-human antibodies against GALR1 (Cat. No. GTX108207, GeneTex, Irvine, CA, USA), GALR2 (Cat. No. GTX100382, GeneTex, Irvine, CA, USA) and GALR3 (Cat. No. GTX10816, GeneTex, Irvine, CA, USA), all diluted 1:400 in PBS.

Techniques:

Journal: Biomolecules

Article Title: Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters

doi: 10.3390/biom12121769

Figure Lengend Snippet: Immunolocalization of galanin receptors (GALR1, GALR2, and GALR3) in myenteric plexuses (MP) close and distant to tumour tissue of CRCpatients (n = 5). Neurons expressing respective GALRs are marked by arrows. Total magnification: 400× g .

Article Snippet: The sections were incubated overnight at 4 °C with rabbit polyclonal anti-human antibodies against GALR1 (Cat. No. GTX108207, GeneTex, Irvine, CA, USA), GALR2 (Cat. No. GTX100382, GeneTex, Irvine, CA, USA) and GALR3 (Cat. No. GTX10816, GeneTex, Irvine, CA, USA), all diluted 1:400 in PBS.

Techniques: Expressing

Journal: Biomolecules

Article Title: Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters

doi: 10.3390/biom12121769

Figure Lengend Snippet: Kaplan–Meier diagrams that show the relative immunoexpression of galanin receptors (GALR1—( A ), GALR2—( B ), and GALR3—( C )) in myenteric plexuses (MP) regarding the overall survival of colorectal cancer (CRC) patients (n = 31).

Article Snippet: The sections were incubated overnight at 4 °C with rabbit polyclonal anti-human antibodies against GALR1 (Cat. No. GTX108207, GeneTex, Irvine, CA, USA), GALR2 (Cat. No. GTX100382, GeneTex, Irvine, CA, USA) and GALR3 (Cat. No. GTX10816, GeneTex, Irvine, CA, USA), all diluted 1:400 in PBS.

Techniques:

Journal: Biomolecules

Article Title: Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters

doi: 10.3390/biom12121769

Figure Lengend Snippet: Analysis of overall survival of CRC patients in relation to GALRs immunoexpression in myenteric plexuses. HR-hazard ratio; CI-confidence interval; MP-myenteric plexus.

Article Snippet: The sections were incubated overnight at 4 °C with rabbit polyclonal anti-human antibodies against GALR1 (Cat. No. GTX108207, GeneTex, Irvine, CA, USA), GALR2 (Cat. No. GTX100382, GeneTex, Irvine, CA, USA) and GALR3 (Cat. No. GTX10816, GeneTex, Irvine, CA, USA), all diluted 1:400 in PBS.

Techniques:

Journal: Biomolecules

Article Title: Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters

doi: 10.3390/biom12121769

Figure Lengend Snippet: Associations between demographic and clinico-pathological features of colorectal cancer patients (n = 31) and relative expression levels of galanin receptors in myenteric plexuses determined by immunohistochemistry.

Article Snippet: The sections were incubated overnight at 4 °C with rabbit polyclonal anti-human antibodies against GALR1 (Cat. No. GTX108207, GeneTex, Irvine, CA, USA), GALR2 (Cat. No. GTX100382, GeneTex, Irvine, CA, USA) and GALR3 (Cat. No. GTX10816, GeneTex, Irvine, CA, USA), all diluted 1:400 in PBS.

Techniques: Expressing, Immunohistochemistry

Journal: Biomolecules

Article Title: Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters

doi: 10.3390/biom12121769

Figure Lengend Snippet: Immunohistochemical expression of galanin receptors (GALR1, GALR2, and GALR3) in submucosal plexuses (SP) distantly located from the colorectal cancer (CRC) tissue compared with their expression in the submucosal plexuses in the vicinity of cancer invasion of the same, representative CRC patients (n = 32). Neurons expressing respective GALRs are marked by arrows. Total magnification: 400× g .

Article Snippet: The sections were incubated overnight at 4 °C with rabbit polyclonal anti-human antibodies against GALR1 (Cat. No. GTX108207, GeneTex, Irvine, CA, USA), GALR2 (Cat. No. GTX100382, GeneTex, Irvine, CA, USA) and GALR3 (Cat. No. GTX10816, GeneTex, Irvine, CA, USA), all diluted 1:400 in PBS.

Techniques: Immunohistochemical staining, Expressing

Journal: Biomolecules

Article Title: Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters

doi: 10.3390/biom12121769

Figure Lengend Snippet: Immunoreactivity (IR) of galanin receptors (GALR1—( A ), GALR2—( C ), and GALR3—( E )) in submucosal plexuses (SP) distantly and in proximally located to cancer invasion in individual colorectal (CRC) patients. The average immunoreactivities of GALRs (GALR1—( B ), GALR2—( D ), and GALR3—( F )) in submucosal plexuses (SP) located distantly from CRC tissue were compared with those in plexuses in the vicinity of cancer cells of colorectal (CRC) patients (n = 31).

Article Snippet: The sections were incubated overnight at 4 °C with rabbit polyclonal anti-human antibodies against GALR1 (Cat. No. GTX108207, GeneTex, Irvine, CA, USA), GALR2 (Cat. No. GTX100382, GeneTex, Irvine, CA, USA) and GALR3 (Cat. No. GTX10816, GeneTex, Irvine, CA, USA), all diluted 1:400 in PBS.

Techniques:

Journal: Biomolecules

Article Title: Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters

doi: 10.3390/biom12121769

Figure Lengend Snippet: Immunolocalization of galanin receptors (GALR1, GALR2, and GALR3) in submucosal plexuses (SP) close and distant to tumour tissue of colorectal cancer patients (n = 5). Neurons expressing respective GALRs are marked by arrows. Total magnification: 400× g .

Article Snippet: The sections were incubated overnight at 4 °C with rabbit polyclonal anti-human antibodies against GALR1 (Cat. No. GTX108207, GeneTex, Irvine, CA, USA), GALR2 (Cat. No. GTX100382, GeneTex, Irvine, CA, USA) and GALR3 (Cat. No. GTX10816, GeneTex, Irvine, CA, USA), all diluted 1:400 in PBS.

Techniques: Expressing

Journal: Biomolecules

Article Title: Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters

doi: 10.3390/biom12121769

Figure Lengend Snippet: Kaplan–Meier diagrams that show the relative immunoexpression of galanin receptors (GALR1—( A ), GALR2—( B ), and GALR3—( C )) in submucosal plexuses (SP) regarding the overall survival of colorectal cancer (CRC) patients (n = 32).

Article Snippet: The sections were incubated overnight at 4 °C with rabbit polyclonal anti-human antibodies against GALR1 (Cat. No. GTX108207, GeneTex, Irvine, CA, USA), GALR2 (Cat. No. GTX100382, GeneTex, Irvine, CA, USA) and GALR3 (Cat. No. GTX10816, GeneTex, Irvine, CA, USA), all diluted 1:400 in PBS.

Techniques:

Journal: Biomolecules

Article Title: Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters

doi: 10.3390/biom12121769

Figure Lengend Snippet: Analysis of overall survival of CRC patients in relation to GALRs immunoexpression in submucosal plexuses. HR-hazard ratio; CI-confidence interval; SP-submucosal plexus.

Article Snippet: The sections were incubated overnight at 4 °C with rabbit polyclonal anti-human antibodies against GALR1 (Cat. No. GTX108207, GeneTex, Irvine, CA, USA), GALR2 (Cat. No. GTX100382, GeneTex, Irvine, CA, USA) and GALR3 (Cat. No. GTX10816, GeneTex, Irvine, CA, USA), all diluted 1:400 in PBS.

Techniques:

Journal: Biomolecules

Article Title: Galanin Receptors (GALR1, GALR2, and GALR3) Immunoexpression in Enteric Plexuses of Colorectal Cancer Patients: Correlation with the Clinico-Pathological Parameters

doi: 10.3390/biom12121769

Figure Lengend Snippet: Association between demographic and clinico-pathological features of colorectal (CRC) patients (n = 32) and relative expression levels of galanin receptors in submucosal plexuses determined by immunohistochemistry.

Article Snippet: The sections were incubated overnight at 4 °C with rabbit polyclonal anti-human antibodies against GALR1 (Cat. No. GTX108207, GeneTex, Irvine, CA, USA), GALR2 (Cat. No. GTX100382, GeneTex, Irvine, CA, USA) and GALR3 (Cat. No. GTX10816, GeneTex, Irvine, CA, USA), all diluted 1:400 in PBS.

Techniques: Expressing, Immunohistochemistry

Journal: Frontiers in Oncology

Article Title: Combined clinical features and MRI parameters for the prediction of VEGFR2 in hepatocellular carcinoma patients

doi: 10.3389/fonc.2022.961530

Figure Lengend Snippet: Flowchart of patient selection. HCC, hepatocellular carcinoma; MRI, contrast-enhanced magnetic resonance imaging; FLL, focal liver lesion; VEGFR2, vascular endothelial growth factor receptor 2.

Article Snippet: Slides were incubated with primary antibodies against human VEGFR2 (1:500, ZSGB-BIO, Beijing, China, ZA-0287) for 60 minutes at 37°C.

Techniques: Selection, Magnetic Resonance Imaging

Journal: Frontiers in Oncology

Article Title: Combined clinical features and MRI parameters for the prediction of VEGFR2 in hepatocellular carcinoma patients

doi: 10.3389/fonc.2022.961530

Figure Lengend Snippet: Characteristics of the 206 patients stratified by the VEGFR2 status of HCC.

Article Snippet: Slides were incubated with primary antibodies against human VEGFR2 (1:500, ZSGB-BIO, Beijing, China, ZA-0287) for 60 minutes at 37°C.

Techniques: Infection

Journal: Frontiers in Oncology

Article Title: Combined clinical features and MRI parameters for the prediction of VEGFR2 in hepatocellular carcinoma patients

doi: 10.3389/fonc.2022.961530

Figure Lengend Snippet: Univariate logistic regression analyses for the prediction of VEGFR2-positive HCC.

Article Snippet: Slides were incubated with primary antibodies against human VEGFR2 (1:500, ZSGB-BIO, Beijing, China, ZA-0287) for 60 minutes at 37°C.

Techniques:

Journal: Frontiers in Oncology

Article Title: Combined clinical features and MRI parameters for the prediction of VEGFR2 in hepatocellular carcinoma patients

doi: 10.3389/fonc.2022.961530

Figure Lengend Snippet: Multivariate logistic regression analyses for the prediction of VEGFR2-positive HCC.

Article Snippet: Slides were incubated with primary antibodies against human VEGFR2 (1:500, ZSGB-BIO, Beijing, China, ZA-0287) for 60 minutes at 37°C.

Techniques:

Journal: Frontiers in Oncology

Article Title: Combined clinical features and MRI parameters for the prediction of VEGFR2 in hepatocellular carcinoma patients

doi: 10.3389/fonc.2022.961530

Figure Lengend Snippet: MRI of a VEGFR2-positive HCC patient with LI-RADS 5. Patient summary: a 50-year-old man with HCC in the left lobe of the liver, AFP level of 266ng/ml, lesion dimension on MRI of 8.1cm, and LI-RADS score of 5. (A) Axial T2-weighted MRI, (B) Axial T1-weighted MRI. (C, D) Early (15s) and late arterial phase (25s). (E) Portal venous phase (1min). (F) Delay phase (3min). (G) Hepatobiliary phase (15min). (H) Axial diffusion-weighted MRI. (I) VEGFR2-positivity in pathology. MRI, magnetic resonance imaging; LI-RADS, liver imaging reporting and data system; AFP, alpha-fetoprotein; VEGFR2, vascular endothelial growth factor receptor 2.

Article Snippet: Slides were incubated with primary antibodies against human VEGFR2 (1:500, ZSGB-BIO, Beijing, China, ZA-0287) for 60 minutes at 37°C.

Techniques: Diffusion-based Assay, Magnetic Resonance Imaging, Imaging

Journal: Frontiers in Oncology

Article Title: Combined clinical features and MRI parameters for the prediction of VEGFR2 in hepatocellular carcinoma patients

doi: 10.3389/fonc.2022.961530

Figure Lengend Snippet: MRI of a VEGFR2-negative HCC patient with LI-RADS 3. Patient summary: a 59-year-old man with HCC in the right lobe of the liver, AFP level 2.7ng/ml, lesion dimension on MRI 3.8cm, and LI-RADS score of 3. (A) Axial T2-weighted MRI. (B) Axial T1-weighted MRI. (C, D) Early (15s) and late arterial phase (25s). (E) Portal venous phase (1min). (F) Delay phase (3min). (G) Hepatobiliary phase (15min). (H) Axial diffusion-weighted MRI. (I) VEGFR2-negative in pathology. MRI, magnetic resonance imaging; LI-RADS, liver imaging reporting and data system; AFP, alpha-fetoprotein; VEGFR2, vascular endothelial growth factor receptor 2.

Article Snippet: Slides were incubated with primary antibodies against human VEGFR2 (1:500, ZSGB-BIO, Beijing, China, ZA-0287) for 60 minutes at 37°C.

Techniques: Diffusion-based Assay, Magnetic Resonance Imaging, Imaging

Journal: Frontiers in Oncology

Article Title: Combined clinical features and MRI parameters for the prediction of VEGFR2 in hepatocellular carcinoma patients

doi: 10.3389/fonc.2022.961530

Figure Lengend Snippet: VEGFR2-positive-risk nomogram establishment and validation. (A) Risk nomogram incorporating AFP and the LI-RADS score for predicting high VEGFR2 expression in HCC patients. (B) ROC curve for the nomogram’s VEGFR2-positive prediction AUC, sensitivity, and specificity. (C) Calibration curves illustrating the nomogram’s calibration in terms of the agreement between the predicted risk of high VEGFR2 expression and the actual pathological VEGFR2 expression status. The 45˚blue line represents a perfect prediction, the red dashed line shows the nomogram’s predictive performance, and the black solid line is bias-corrected. The nomogram’s forecast accuracy improves as the dashed line approaches the ideal line. AUC, area under the curve; AFP, alpha-fetoprotein, LI-RADS, Liver Imaging Reporting and Data System, VEGFR2, vascular endothelial growth factor receptor 2, ROC, receiver operating characteristic.

Article Snippet: Slides were incubated with primary antibodies against human VEGFR2 (1:500, ZSGB-BIO, Beijing, China, ZA-0287) for 60 minutes at 37°C.

Techniques: Biomarker Discovery, Expressing, Imaging

Journal: Frontiers in Oncology

Article Title: Combined clinical features and MRI parameters for the prediction of VEGFR2 in hepatocellular carcinoma patients

doi: 10.3389/fonc.2022.961530

Figure Lengend Snippet: Decision curve analysis for the VEGFR2-positive-risk nomogram. The red line represents the nomogram, the blue line represents the condition that all patients are VEGFR2-positive, and the black line represents the condition that no patient harbors detectable VEGFR2 expression. The decision curve reveals that utilizing the VEGFR2-positive-risk model to forecast VEGFR2 expression offers a greater benefit than intervening in either all or none of the patients when the threshold probability ranges from 0.07 to 0.95.

Article Snippet: Slides were incubated with primary antibodies against human VEGFR2 (1:500, ZSGB-BIO, Beijing, China, ZA-0287) for 60 minutes at 37°C.

Techniques: Expressing

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: The functional role of hemojuvelin in acute ischemic stroke

doi: 10.1177/0271678X19861448

Figure Lengend Snippet: Cells immunoreactive to HJV were found in human brain tissues and were more abundant in stroke patients (n = 10) than controls (n = 2). (a) Immunohistochemical staining of HJV from cortex region of normal (n = 2) and acute stroke patient (n = 10). (b) Characterization of cells expressing HJV in the stroke patient brain section (n = 4) by double immunofluorescence with anti-MAP2 or anti-IBA1 antibodies. (c) Double immunofluorescence micrographs of HJV and HO-1 from cortex region of normal (n=2) and acute stroke patients (n = 4). Scale bars: 50 μm. (d) Percentage of HJV-positive cells, HO-1-positive cells or double-positive cells among total cells from biopsy specimen. Data were expressed as mean±SD; ***, P<0.001 vs. control group. (e) Schematic of the clinical human brain tissue in reference to the infarction region (Red color).

Article Snippet: Brain sections were processed for immunofluorescence staining or IHC with a non-biotin-amplified method (Novocastra Laboratories Ltd, Newcastle Upon Tyne, UK) using primary antibodies against human HJV (Abnova).

Techniques: Immunohistochemical staining, Staining, Expressing, Immunofluorescence, Control

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: The functional role of hemojuvelin in acute ischemic stroke

doi: 10.1177/0271678X19861448

Figure Lengend Snippet: Time-course of HJV/Hepcidin axis in mice after receiving middle cerebral artery occlusion. (a) Brains of stroke mice were dissected at 3, 24, 72 h after MCAO surgery and compared with sham group. Representative Western immunoblots for HJV, Hepcidin, and HO-1 in mice brain at indicated time point. Quantification of immunoblot band intensity of brain lysates for each time point. Intensities of bands were normalized by β-actin, which was used as loading controls. n = 7 in sham, post-I/R 3, or 72 h group. n = 6 in post-I/R 24 h group. (b) Bloods from stroke mice after immunoprecipitation with HJV antibody were analyzed by immunoblotting. Intensities of bands were normalized by anti-mice serum, which was used as loading control. Molecular size markers are indicated at the left in kDa. Data were expressed as mean±SD; *, P < 0.05; **, P < 0.01 versus sham group. (c) Immunofluorescence staining of HJV in mice brain sections revealed significantly increased HJV expression in Mice brains from C57BL/6 mice suffered 1 h MCAO surgery and 72 h reperfusion (n = 2 in each group).

Article Snippet: Brain sections were processed for immunofluorescence staining or IHC with a non-biotin-amplified method (Novocastra Laboratories Ltd, Newcastle Upon Tyne, UK) using primary antibodies against human HJV (Abnova).

Techniques: Western Blot, Immunoprecipitation, Control, Immunofluorescence, Staining, Expressing

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: The functional role of hemojuvelin in acute ischemic stroke

doi: 10.1177/0271678X19861448

Figure Lengend Snippet: HJV was increased in mice neuron cells under OGD condition. (a) Mice neuron cells under OGD for different time points. Cells were fixed and stained with anti-HJV (green) and DPAI (blue). Scale bars: 50 μm. (b) Cell viability of mice neuron cells was performed by CCK-8 method. Cells transfected with scRNA or siHJV to knockdown the expression of HJV under OGD treatment at a designated time point. (c) Representative Western immunoblots for HJV and phosphorylated JNK from mice neuron cells under reoxygenation condition at indicated time point after OGD treatment. (d) Intensities of bands were normalized by β-actin, which was used as loading control. Molecular size markers were indicated at the left in kDa. Data were expressed as mean ± SD, n = 3. *P < 0.05; ***, P < 0.001; N.S., no significant versus control group.

Article Snippet: Brain sections were processed for immunofluorescence staining or IHC with a non-biotin-amplified method (Novocastra Laboratories Ltd, Newcastle Upon Tyne, UK) using primary antibodies against human HJV (Abnova).

Techniques: Staining, CCK-8 Assay, Transfection, Knockdown, Expressing, Western Blot, Control